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KMID : 0613820050150060909
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Journal of Life Science
2005 Volume.15 No. 6 p.909 ~ p.915
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Biogenesis of Epstein-Barr Virus MicroRNAs in B Cells
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Kim Do-Nyun
Oh Sang-Taek
Lee Jae-Myun
Lee Won-Keun
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Abstract
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We investigated microRNA (miRNA) biogenesis of Epstein-Barr virus (EBV) which is the first virus shown to produce viral miRNAs. As expected, expression of all the reported EBV miRNAs were detected by Northen blot in an EBV-infected B cell line, B95-8; BHRF1-1, BHRF1-2, BHRF1-3, BART1, and BART2. The putative EBV pri-miRNAs and pre-miRNAs predicted from the known mature EBV miRNA sequences were detected by RT-PCR in B95-8 cells. Many animal miRNA genes exist as clusters of 2-7 genes and they are expressed polycistronically. As the EBV miRNAs are clustered in two regions of the EBV genome, we examined whether these clustered EBV miRNA genes are also expressed polycistronically. A long polycistronic transcript with the expected size (1602 bp) corresponding to the BHRF1-1～BHRF1-2～BHRF1-3 was amplified. However, any polycistronic transcript containing both BART1 and BART2 was detectable in B95-8. These results suggest that EBV miRNAs may be processed in a similar way with animal miRNAs and that some of the clustered EBV miRNAs can be transcribed polycistronically.
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